Effects of plasticisers and related compounds on the expression of the soluble form of catechol-o-methyltransferase in MCF-7 cells

P. W.L. Ho; A. C.Y. Chu; K. H.H. Kwok; H. F. Liu; M. H.W. Kung; D. B. Ramsden; S. L. Ho

doi:10.2174/138920008784220628

Effects of plasticisers and related compounds on the expression of the soluble form of catechol-o-methyltransferase in MCF-7 cells

P. W.L. Ho, A. C.Y. Chu, K. H.H. Kwok, H. F. Liu, M. H.W. Kung, D. B. Ramsden, S. L. Ho

Research output: Journal article publication › Review article › Academic research › peer-review

2 Citations (Scopus)

Abstract

Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

Original language	English
Pages (from-to)	276-279
Number of pages	4
Journal	Current Drug Metabolism
Volume	9
Issue number	4
DOIs	https://doi.org/10.2174/138920008784220628
Publication status	Published - May 2008
Externally published	Yes

Keywords

COMT
Endocrine disruption
Estrogen
Phthalate
Plasticiser

ASJC Scopus subject areas

Pharmacology
Clinical Biochemistry

More information

10.2174/138920008784220628

Cite this

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title = "Effects of plasticisers and related compounds on the expression of the soluble form of catechol-o-methyltransferase in MCF-7 cells",

abstract = "Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.",

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author = "Ho, {P. W.L.} and Chu, {A. C.Y.} and Kwok, {K. H.H.} and Liu, {H. F.} and Kung, {M. H.W.} and Ramsden, {D. B.} and Ho, {S. L.}",

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AU - Ho, P. W.L.

AU - Chu, A. C.Y.

AU - Kwok, K. H.H.

AU - Liu, H. F.

AU - Kung, M. H.W.

AU - Ramsden, D. B.

AU - Ho, S. L.

PY - 2008/5

Y1 - 2008/5

N2 - Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

AB - Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tertoctyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ERα receptor antagonist ICI182780. 4-(tert-octyl)phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

KW - COMT

KW - Endocrine disruption

KW - Estrogen

KW - Phthalate

KW - Plasticiser

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M3 - Review article

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SN - 1389-2002

VL - 9

SP - 276

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ER -

Effects of plasticisers and related compounds on the expression of the soluble form of catechol-o-methyltransferase in MCF-7 cells

Abstract

Keywords

ASJC Scopus subject areas

More information

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