Effects of organophosphorus pesticides and their ozonation byproducts on gap junctional intercellular communication in rat liver cell line

Jiguo Wu, Li Lin, Tiangang Luan, Yuk Sing Gilbert Chan, Chongyu Lan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)


The effects of organophosphorus pesticides (OPs), oxons and their ozonation byproducts on gap junctional intercellular communication (GJIC) on cultured BRL cell line were investigated using scrape loading and dye transfer (SL/DT) technique. The neutral red uptake assay was used to identify the non-cytotoxic levels of diazinon, parathion and methyl-parathion applied to GJIC assay. The concentration-dependent inhibition of GJIC was observed over a range of 50-350 mg/l diazinon, parathion and methyl-parathion after 90 min incubation compared with the vehicle control. However, oxons and ozonation byproducts of OPs had no inhibition effect on GJIC at any of the concentrations tested. The inhibition of GJIC by OPs was reversible after removal of the tested pesticides followed by incubation with fresh medium. The present study suggested that the ozonation treatment could be used for the detoxification of drinking water and food crops contaminated with diazinon, parathion and methyl-parathion without formation of GJIC toxicity.
Original languageEnglish
Pages (from-to)2057-2063
Number of pages7
JournalFood and Chemical Toxicology
Issue number10
Publication statusPublished - 1 Oct 2007


  • Buffalo rat liver
  • Diazinon
  • Methyl-parathion
  • Ozone
  • Parathion

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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