Effect of mixing time on taxoid production using suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor

Jian Jiang Zhong, Zhi Wei Pan, Zhen Yu Wang, Jianyong Wu, Feng Chen, Mutsumi Takagi, Toshiomi Yoshida

Research output: Journal article publicationJournal articleAcademic researchpeer-review

31 Citations (Scopus)

Abstract

The effects of fluid mixing on the cell growth and secondary metabolite production of plant cells were investigated in a low-shear centrifugal impeller bioreactor (CIB) system. Suspension cultures of Taxus chinensis cells producing taxuyunnanine C (Tc), a physiologically active secondary metabolite, were used as a model system for this investigation. The mixing time (tm) and volumetric oxygen transfer coefficient (kLa) in the bioreactor were characterized at various cell densities and operating conditions. A constant tmof 5 s or 10 s was maintained during cultivation by adjusting the impeller agitation speed with no detrimental effect on the cultured cells. A higher cell density, Tc content and total Tc production were obtained under the shorter mixing time of 5 s. The favorable effect of more rapid mixing on Tc production was also confirmed when the Tc accumulation was significantly increased through culture elicitation using 100 μM methyl jasmonate (MJA). The lower Tc production at the longer tmof 10 s was mainly attributed to oxygen transfer limitation in the dead zones and larger cell aggregates resulting from poor mixing.
Original languageEnglish
Pages (from-to)244-250
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume94
Issue number3
DOIs
Publication statusPublished - 1 Jan 2002

Keywords

  • Centrifugal impeller bioreactor
  • Mixing time
  • Oxygen transfer
  • Plant cell culture
  • Taxoid production
  • Taxus chinensis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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