Effect of flickering light stimulation on full-field electroretinography in mice

Milan Rai, Yamunadevi Lakshmanan, Ho Lung Henry Chan

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review


Purpose : Flicker-induced retinal stimulation has been found to increase the retinal blood flow in response to increased metabolic demands of activated retinal neurons, but the influence of flickering light exposure on full-field electroretinogram (ffERG) is still unclear. The purpose of this study was to investigate the physiological changes of retinal response by ffERG after brief flickering light stimulation.

Methods : Nine anaesthetized C57BL/6J mice (10 week-old) were light adapted at 1 cd/m2 for 10 minutes. After light adaptation, baseline ffERG (pre-flickering) was recorded and then was followed by 60 seconds of resting period to eliminate the aftereffect of light flashes. After resting period, the tested eye was stimulated by 8-Hz flickering light with 0.20 cd.s/m2 for 60 seconds that was immediately followed by post-flickering ffERG recording. Amplitudes and implicit times of ERG responses measured before and after flickering light stimulation were compared by paired t-test.

Results : Retinal flickering light stimulation induced a significant increase in b-wave amplitude (pre-flickering amplitude=170.50±11.38μV; post-flickering amplitude=187.79±12.37μV; percentage change=10.42%±5.71%, p0.05), a-wave amplitude (pre-flickering amplitude=-43.50±7.07μV; post-flickering amplitude=-36.95±6.51μV; percentage change=-11.91%±56.37%, p>0.05) and a-wave implicit time (pre-flickering implicit time=22.08±1.11ms; post-flickering implicit time=21.87±1.21ms; percentage change=-1.12%±6.13%, p>0.05).

Conclusions : Flickering light stimulation on retina led to a significant increase in ERG responses originating from middle retinal layer, primarily from bipolar cells but did not have significant effect on responses originating from photoreceptors. Further studies are required to understand the mechanism underlying enhanced ERG responses related to flicker-induced hyperemia.
Original languageEnglish
Title of host publicationnvestigative Ophthalmology & Visual Science
ISBN (Electronic)1552-5783
Publication statusPublished - Jun 2022
EventAssociation for Research in Vision and Ophthalmology (ARVO) Annual Meeting 2022 - Denver, United States
Duration: 1 May 20224 May 2022


ConferenceAssociation for Research in Vision and Ophthalmology (ARVO) Annual Meeting 2022
Abbreviated titleARVO 2022
Country/TerritoryUnited States


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