Dynamics of oxygen level-driven regulators in modulating autophagy in colorectal cancer cells

Shing Yau Tam, Vincent W.C. Wu, Helen K.W. Law

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)

Abstract

Colorectal cancer is a common cancer with metachronous distant metastases still threatening overall survival. Tumor oxygen level influences tumor radiosensitivity in relation to autophagy and apoptosis. The objective of this study is to evaluate the expression and interaction between multiple key regulators in different oxygen levels. Human colorectal adenocarcinoma HT-29 cells were cultured in 1% or 10% oxygen level and irradiated by 2 Gy with different incubation time. Autophagy key regulators, AMPK, HIFs and JNK were evaluated by Western blot. Sequential autophagy key regulator activation was observed in the order of AMPK, HIF-1α, HIF-2α and JNK. 10% oxygen level could promote autophagy with similar degree of autophagy activation as 1% oxygen level in 48-h while irradiation could slightly inhibit autophagy. The results of this study supported prior evaluation of oxygen level and autophagy regulators for improving treatment efficacy and indicated the possible directions in developing individualized radiotherapy by selective targeting of hypoxic regions.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume517
Issue number2
DOIs
Publication statusPublished - 17 Sep 2019

Keywords

  • Autophagy
  • Autophagy regulator
  • Colorectal cancer cell
  • Hypoxia
  • Oxygen level

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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