Dynamics of mitochondria and mitochondrial Ca2+ near the plasma membrane of PC12 cells: A study by multimode microscopy

De Ming Yang, Chung Chih Lin, Hsia Yu Lin, Chien Chang Huang, Din Ping Tsai, Chin Wen Chi, Lung Sen Kao

Research output: Journal article publicationJournal articleAcademic researchpeer-review

2 Citations (Scopus)


The goal of this study is to examine whether there is a difference in the regulation of Ca2+ between mitochondria near the cell surface and mitochondria in the cytosol. Total internal reflection fluorescence and epifluorescence microscopy were used to monitor changes in the mitochondrial Ca2+ ([Ca2+]mt,) between the mitochondria near the plasma membrane and those in the cytosol. The results show that [Ca 2+]mt near the plasma membrane increased earlier and decayed slower after high K+ stimulation than average mitochondria in the cytosol. In addition, the changes in [Ca2+]mt in the mitochondria near the cell surface after a second stimulation were larger than those induced by the first stimulation. The results provide direct evidence to support the hypothesis that mitochondria in different subcellular localization show differential responses to the influx of extracellular Ca2+.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalAnnals of the New York Academy of Sciences
Publication statusPublished - 1 Jan 2005
Externally publishedYes


  • Mitochondria
  • Mitochondrial Ca
  • PC12 cells
  • Total internal reflection fluorescence microscopy

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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