Abstract
Human promyelocytic leukemia HL-60 cells were induced to undergo granulocytic differentiation by treatment with retinoic acid (RA, 10 μM, 1-5 days). The steady-state level of nucleophosmin/B23 mRNA decreased during the RA-induced differentiation. There was also decrease in the level of total cellular nucleophosmin/B23 protein during the RA-induced differentiation. Stabilization and nuclear run-on assays indicate that the decrease in nucleophosmin/B23 mRNA in RA-treated HL-60 cells was transcriptionally regulated. Unlike c-myc mRNA, there was virtually no decline of nucleophosmin/B23 mRNA during the growth arrest by serum-starvation. The decrease in nucleophosmin/B23 mRNA expression in HL-60 cells subsequent to retinoic acid treatment can thus be attributed to cellular differentiation rather than the growth arrest induced by RA. Nucleophosmin/B23 antisense oligomer treatment significantly potentiated RA-induced cellular differentiation. Results of this study suggest that nucleophosmin/B23 is one of the key elements in the down-regulation of nucleolar function for cellular differentiation.
Original language | English |
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Pages (from-to) | 915-923 |
Number of pages | 9 |
Journal | Oncogene |
Volume | 16 |
Issue number | 7 |
DOIs | |
Publication status | Published - 19 Feb 1998 |
Externally published | Yes |
Keywords
- HL-60 differentiation
- Nucleophosmin/B23
- Retinoic acid
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research