DNA polymorphism at the ABO locus in the Chinese population of Hong Kong

Shea Ping Yip; C. M.N. Yow; W. H.P. Lewis

doi:10.1159/000154311

DNA polymorphism at the ABO locus in the Chinese population of Hong Kong

Shea Ping Yip, C. M.N. Yow, W. H.P. Lewis

Research output: Journal article publication › Journal article › Academic research › peer-review

15 Citations (Scopus)

Abstract

DNA polymorphism at the ABO locus was investigated using denaturing gradient gel electrophoresis of polymerase-chain-reaction-amplified DNA products from 315 healthy individuals of the Chinese population of Hong Kong. Five different alleles were identified: ABO*O1, O2, A1, A2 and B1. The ABO*A2 is a new allele not previously reported in the literature. The allele frequencies found were 0.3698 for O1, 0.2508 for O2, 0.1952 for A1, 0.0159 for A2, and 0.1683 for B1. The Chinese population being investigated was in Hardy-Weinberg equilibrium. The genotyping method identifies many more alleles in the ABO locus and thus makes it a more useful genetic marker in linkage analysis, paternity testing and individualization in forensic work than when the locus is genotyped using conventional DNA-based methods or phenotyped using serological methods.

Original language	English
Pages (from-to)	266-271
Number of pages	6
Journal	Human Heredity
Volume	45
Issue number	5
DOIs	https://doi.org/10.1159/000154311
Publication status	Published - 1 Jan 1995

Keywords

ABO gene
Chinese population
Denaturing gradient gel electrophoresis
DNA polymorphism
Hong Kong
Polymerase chain reaction

ASJC Scopus subject areas

Genetics
Genetics(clinical)

More information

10.1159/000154311

Cite this

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title = "DNA polymorphism at the ABO locus in the Chinese population of Hong Kong",

abstract = "DNA polymorphism at the ABO locus was investigated using denaturing gradient gel electrophoresis of polymerase-chain-reaction-amplified DNA products from 315 healthy individuals of the Chinese population of Hong Kong. Five different alleles were identified: ABO*O1, O2, A1, A2 and B1. The ABO*A2 is a new allele not previously reported in the literature. The allele frequencies found were 0.3698 for O1, 0.2508 for O2, 0.1952 for A1, 0.0159 for A2, and 0.1683 for B1. The Chinese population being investigated was in Hardy-Weinberg equilibrium. The genotyping method identifies many more alleles in the ABO locus and thus makes it a more useful genetic marker in linkage analysis, paternity testing and individualization in forensic work than when the locus is genotyped using conventional DNA-based methods or phenotyped using serological methods.",

keywords = "ABO gene, Chinese population, Denaturing gradient gel electrophoresis, DNA polymorphism, Hong Kong, Polymerase chain reaction",

author = "Yip, {Shea Ping} and Yow, {C. M.N.} and Lewis, {W. H.P.}",

year = "1995",

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AU - Yip, Shea Ping

AU - Yow, C. M.N.

AU - Lewis, W. H.P.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - DNA polymorphism at the ABO locus was investigated using denaturing gradient gel electrophoresis of polymerase-chain-reaction-amplified DNA products from 315 healthy individuals of the Chinese population of Hong Kong. Five different alleles were identified: ABO*O1, O2, A1, A2 and B1. The ABO*A2 is a new allele not previously reported in the literature. The allele frequencies found were 0.3698 for O1, 0.2508 for O2, 0.1952 for A1, 0.0159 for A2, and 0.1683 for B1. The Chinese population being investigated was in Hardy-Weinberg equilibrium. The genotyping method identifies many more alleles in the ABO locus and thus makes it a more useful genetic marker in linkage analysis, paternity testing and individualization in forensic work than when the locus is genotyped using conventional DNA-based methods or phenotyped using serological methods.

AB - DNA polymorphism at the ABO locus was investigated using denaturing gradient gel electrophoresis of polymerase-chain-reaction-amplified DNA products from 315 healthy individuals of the Chinese population of Hong Kong. Five different alleles were identified: ABO*O1, O2, A1, A2 and B1. The ABO*A2 is a new allele not previously reported in the literature. The allele frequencies found were 0.3698 for O1, 0.2508 for O2, 0.1952 for A1, 0.0159 for A2, and 0.1683 for B1. The Chinese population being investigated was in Hardy-Weinberg equilibrium. The genotyping method identifies many more alleles in the ABO locus and thus makes it a more useful genetic marker in linkage analysis, paternity testing and individualization in forensic work than when the locus is genotyped using conventional DNA-based methods or phenotyped using serological methods.

KW - ABO gene

KW - Chinese population

KW - Denaturing gradient gel electrophoresis

KW - DNA polymorphism

KW - Hong Kong

KW - Polymerase chain reaction

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DO - 10.1159/000154311

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DNA polymorphism at the ABO locus in the Chinese population of Hong Kong

Abstract

Keywords

ASJC Scopus subject areas

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