Diterpenoid tanshinones and phenolic acids from cultured hairy roots of salvia miltiorrhiza bunge and their antimicrobial activities

Jianglin Zhao, Jingfeng Lou, Yan Mou, Peiqin Li, Jianyong Wu, Ligang Zhou

Research output: Journal article publicationJournal articleAcademic researchpeer-review

62 Citations (Scopus)


Four diterpenoid tanshinones and three phenolic acids were isolated from the crude ethanol extract of the cultured hairy roots of Salvia miltiorrhiza Bunge by bioassayguided fractionation. By means of physicochemical and spectrometric analysis, they were identified as tanshinone IIA (1), tanshinone I (2), cryptotanshinone (3), dihydrotanshinone I (4), rosmarinic acid (5), caffeic acid (6), and danshensu (7). These compounds were evaluated to show a broad antimicrobial spectrum of activity on test microorganisms including eight bacterial and one fungal species. Among the four tanshinones, cryptotanshinone (3) and dihydrotanshinone I (4) exhibited stronger antimicrobial activity than tanshinone IIA (1) and tanshinone I (2). The results indicated that the major portion of the antimicrobial activity was due to the presence of tanshinones and phenolic acids in S. miltiorrhiza hairy roots, which could be used as the materials for producing antimicrobial agents for use in agricultural practice in the future.
Original languageEnglish
Pages (from-to)2259-2267
Number of pages9
Issue number3
Publication statusPublished - 1 Mar 2011


  • Antimicrobial activity
  • Cultured hairy roots
  • Diterpenoid tanshinones
  • Phenolic acids
  • Salvia miltiorrhiza Bunge

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

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