Distribution of the neurotransmitters in the retina of Wistar and RCS rats

Feng Pan, Wei WANG (Corresponding Author), Huirong Zhang, Debo You, Li Shen, Lei Yang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Objective: To determine the localization and extent of expression of two neurotransmitters (Glutamate, γ-aminobutyrate, GABA) in the retinas of Wistar and Royal College of Surgeons (RCS) rats. Methods: Tissue sections were stained by immunohistochemistry and observed using the light microscopy. The ratinas of the 74-days old healthy Wistar and RCS rats were divided and processed for light microscopic analysis. The quantity of the amacrine cells and the relative density of neurofibrils in the inner plexiform layer (IPL) and ganglion cell layer (GCL) of the two groups were determined. Results: In the retina of Wistar rats, GABA-like immunoreactivity was observed in the inner plexiform layer, GABA-ergic amacrine cells and in the ganglion cell layer. Glutamate-like immunoreactivity was observed in the outer plexiform layer, inner plexiform layer and ganglion cell layer. In the retina of RCS rats, GABA-like immunoreactivity was observed in the inner plexiform layer, few GABA-ergic amacrine cells and the ganglion cell layer. Glutamate-like immunoreactivity was observed in the inner plexiform layer and ganglion cell layer. There were significant differences in the relative density of GABA-like immunoreactivity (IR) neurofibrils within the IPL between the RCS rats and the Wistar rats. There were differences in the relative density of the GABA-ergic amacrine cells between the RCS rats and the Wistar rats. Conclusion: These results suggest that the loss of the photoreceptor cells in the 74-day old RCS rats has effects on the amino acid neurotransmitters of the retina.
Original languageChinese (Simplified)
Pages (from-to)138-140
Number of pages3
JournalChinese Ophthalmic Research
Volume19
Issue number2
Publication statusPublished - Apr 2001
Externally publishedYes

Keywords

  • γ-Aminobutyric acid
  • Glutamate

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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