TY - JOUR
T1 - Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles
AU - Chua, Song Lin
AU - Liu, Yang
AU - Yam, Joey Kuok Hoong
AU - Chen, Yicai
AU - Vejborg, Rebecca Munk
AU - Tan, Bryan Giin Chyuan
AU - Kjelleberg, Staffan
AU - Tolker-Nielsen, Tim
AU - Givskov, Michael
AU - Yang, Liang
N1 - Funding Information:
We thank Dr Daniela Drautz, Mr Yap Zhei Hwee and Rikky Wenang Purbojati for help with the RNA-seq experiments. We thank Mr Wu Yichao for his guidance in c-di-GMP quantification. This research is supported by the National Research Foundation and Ministry of Education Singapore under its Research Centre of Excellence Programme and the Start-up Grants (M4330002.C70) from Nanyang Technological University, Singapore. This work was supported by grants from the Danish Council for Strategic Research (M.G.), the Danish Council for Independent Research, and the Lundbeck Foundation.
PY - 2014/7/21
Y1 - 2014/7/21
N2 - Bacteria assume distinct lifestyles during the planktonic and biofilm modes of growth. Increased levels of the intracellular messenger c-di-GMP determine the transition from planktonic to biofilm growth, while a reduction causes biofilm dispersal. It is generally assumed that cells dispersed from biofilms immediately go into the planktonic growth phase. Here we use single-nucleotide resolution transcriptomic analysis to show that the physiology of dispersed cells from Pseudomonas aeruginosa biofilms is highly different from those of planktonic and biofilm cells. In dispersed cells, the expression of the small regulatory RNAs RsmY and RsmZ is downregulated, whereas secretion genes are induced. Dispersed cells are highly virulent against macrophages and Caenorhabditis elegans compared with planktonic cells. In addition, they are highly sensitive towards iron stress, and the combination of a biofilm-dispersing agent, an iron chelator and tobramycin efficiently reduces the survival of the dispersed cells.
AB - Bacteria assume distinct lifestyles during the planktonic and biofilm modes of growth. Increased levels of the intracellular messenger c-di-GMP determine the transition from planktonic to biofilm growth, while a reduction causes biofilm dispersal. It is generally assumed that cells dispersed from biofilms immediately go into the planktonic growth phase. Here we use single-nucleotide resolution transcriptomic analysis to show that the physiology of dispersed cells from Pseudomonas aeruginosa biofilms is highly different from those of planktonic and biofilm cells. In dispersed cells, the expression of the small regulatory RNAs RsmY and RsmZ is downregulated, whereas secretion genes are induced. Dispersed cells are highly virulent against macrophages and Caenorhabditis elegans compared with planktonic cells. In addition, they are highly sensitive towards iron stress, and the combination of a biofilm-dispersing agent, an iron chelator and tobramycin efficiently reduces the survival of the dispersed cells.
UR - http://www.scopus.com/inward/record.url?scp=84904704133&partnerID=8YFLogxK
U2 - 10.1038/ncomms5462
DO - 10.1038/ncomms5462
M3 - Journal article
C2 - 25042103
AN - SCOPUS:84904704133
SN - 2041-1723
VL - 5
JO - Nature Communications
JF - Nature Communications
M1 - 4462
ER -