Direct synthesis of rev peptide-conjugated gold nanoparticles and their application in cancer therapeutics

Ngoc Thi Thanh Tran, Tzu Hao Wang, Chiao Yun Lin, Yi Chun Tsai, Chyong Huey Lai, Yian Tai, Yat Ming Yung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

16 Citations (Scopus)

Abstract

We have developed a simple approach for generating peptide-conjugated gold nanoparticles (AuNPs) from the Rev peptide and gold aqueous solution. The peptide functions as both a reducing agent and a capping molecule. AuNPs of various sizes (20 - 300 nm) and shapes (spheres, triangular plates, and polygons) can be obtained upon modulating the ratio of gold ions to the Rev peptide. Transmission electron microscopy, X-ray diffraction, and UV - vis spectroscopy were utilized to characterize these nanoparticles. Fourier-transform infrared and X-ray photoelectron spectroscopy measurements were performed to investigate chemical interactions between the Rev peptide and AuNPs. Lactate dehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assays revealed that the Rev peptide - AuNP nanocomposites exhibited exceptionally high cytotoxic effects toward mouse ovarian surface epithelial cell lines, relative to the effects of equal doses of the free Rev peptide. Our study suggests a new way of utilizing biomolecule-conjugated AuNPs as potentially effective anticancer drugs.
Original languageEnglish
Pages (from-to)1394-1401
Number of pages8
JournalBioconjugate Chemistry
Volume22
Issue number7
DOIs
Publication statusPublished - 20 Jul 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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