Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay

Kingsley King Gee Tam, Kenneth Siu Sing Leung, Sabrina Wai Chi To, Kit Hang Siu, Terrence Chi Kong Lau, Victor Chi Man Shek, Cindy Wing Sze Tse, Samson Sai Yin Wong, Pak Leung Ho, Wing Cheong Yam

Research output: Journal article publicationJournal articleAcademic researchpeer-review

14 Citations (Scopus)

Abstract

Abbott RealTime MTB (Abbott-RT) in conjunction with Abbott RealTime MTB RIF/INH Resistance (Abbott-RIF/INH) is a new, high-throughput automated nucleic acid amplification platform (Abbott-MDR) for detection of Mycobacterium tuberculosis complex (MTBC) and the genotypic markers for rifampicin (RIF) and isoniazid (INH) resistance directly from respiratory specimens. This prospective study evaluated the diagnostic performance of this new platform for MTBC and multidrug-resistant tuberculosis (MDR-TB) using 610 sputum specimens in a tuberculosis high-burden setting. Using conventional culture results and clinical background as reference standards, Abbott-RT exhibited an overall sensitivity and specificity of 95.2% and 99.8%, respectively. Genotypic RIF/INH resistance of 178 “MTB detected” specimens was subsequently analyzed by Abbott-RIF/INH. Compared to phenotypic drug susceptibility test results, Abbott-RIF/INH detected resistance genotypic markers in 84.6% MDR-TB, 80% mono-RIF-resistant and 66.7% mono-INH-resistant specimens. Two of the RIF-resistant specimens carried a novel single, nonsense mutation at rpoB Q513 and in silico simulation demonstrated that the truncated RpoB protein failed to bind with other subunits for transcription. Overall, Abbott-MDR platform provided high throughput and reliable diagnosis of MDR-TB within a TB high-burden region.
Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume89
Issue number2
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Direct detection
  • Multidrug-resistant tuberculosis
  • Mycobacteriology
  • Mycobacterium tuberculosis
  • Respiratory specimen

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this