Differential cellular distribution of retinoic acid during staurosporine potentiation of retinoic acid-induced granulocytic differentiation in human leukemia HL-60 cells

Yat Ming Yung, Eric Ka Wai Hui

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)


Pretreatment of cells with staurosporine, a protein kinase C (PKC) inhibitor, was found to potentiate the granulocytic differentiation induced by a brief (2 h) retinoic acid treatment. By cell cycle analysis, staurosporine was found to have little effect on the cell cycle. Retinoic acid was distributed equally in the nuclei (40%) and in the plasma membrane (40%) of staurosporine-pretreated cells while less than 20% of retinoic acid was found in the membrane of control non-staurosporine-pretreated cells during the retinoic acid-induced differentiation. These results indicate that the enhancing effect of staurosporine may be somehow associated with the localization of retinoic acid in the plasma membrane of the cell. -1This work is dedicated to Prof. Harris Busch (Baylor College of Medicine, Tex., USA) for his 33 wonderful years at Baylor and 50 years in medicine.
Original languageEnglish
Pages (from-to)154-159
Number of pages6
JournalJournal of Biomedical Science
Issue number2
Publication statusPublished - 1 Apr 1995
Externally publishedYes


  • Induction of differentiation
  • Retinoic acid distribution
  • Staurosporine potentiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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