Different functional types of bipolar cells use different gap-junctional proteins

Bin Lin, Tatjana C. Jakobs, Richard H. Masland

Research output: Journal article publicationJournal articleAcademic researchpeer-review

43 Citations (Scopus)

Abstract

Rod signals are transmitted to ON retinal ganglion cells by means of gap junctions between All amacrine cells and ON bipolars. The All amacrine cells are known to express connexin36 (Cx36), but previous studies of Cx36 in ON cone bipolars have been ambiguous. Here, we studied bipolar cells in a transgenic mouse line that expresses high levels of green fluorescent protein (GFP) in one type of ON cone bipolar cell. We found strong Cx36 immunostaining in the axon terminals of the GFP-labeled type 357 bipolar cells in both vertical sections and whole mounts of the retina. This finding was confirmed by single-cell immunostaining and single-cell reverse transcription-PCR (RT-PCR). As reported previously (Maxeiner et al., 2005), Cx45 was found in some ON bipolar cells, but RT-PCR showed Cx36 and not Cx45 to be expressed by the type 357 bipolar cells. Some of the remaining GFP-negative bipolar cells expressed Cx45 but not Cx36. It appears that different types of ON cone bipolar cells express different connexins at their gap junctions with All amacrine cells.
Original languageEnglish
Pages (from-to)6696-6701
Number of pages6
JournalJournal of Neuroscience
Volume25
Issue number28
DOIs
Publication statusPublished - 13 Jul 2005
Externally publishedYes

Keywords

  • Cx36
  • Gap junctions
  • GFP
  • Mouse
  • ON bipolar cell
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

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