Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish

Ivan Fan Xia, Jacky ST Cheung, Manhui Wu, Kwong Sen Wong, Hang Kin Kong, Xiao Ting Zheng, Ka Hing Wong, Kevin WH Kwok

Research output: Journal article publicationJournal articleAcademic researchpeer-review

26 Citations (Scopus)


Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.

Original languageEnglish
Pages (from-to)449-459
Number of pages11
JournalFish and Shellfish Immunology
Publication statusPublished - 1 Apr 2019


  • Bacteria infection
  • Combined material
  • Immunostimulant
  • Nanoparticles

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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