Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish

Ivan Fan Xia; Jacky ST Cheung; Manhui Wu; Kwong Sen Wong; Hang Kin Kong; Xiao Ting Zheng; Ka Hing Wong; Kevin WH Kwok

doi:10.1016/j.fsi.2019.01.042

Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish

Ivan Fan Xia, Jacky ST Cheung, Manhui Wu, Kwong Sen Wong, Hang Kin Kong, Xiao Ting Zheng, Ka Hing Wong, Kevin WH Kwok

Research output: Journal article publication › Journal article › Academic research › peer-review

53 Citations (Scopus)

Abstract

Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.

Original language	English
Pages (from-to)	449-459
Number of pages	11
Journal	Fish and Shellfish Immunology
Volume	87
DOIs	https://doi.org/10.1016/j.fsi.2019.01.042
Publication status	Published - 1 Apr 2019

Keywords

Bacteria infection
Combined material
Immunostimulant
Nanoparticles

ASJC Scopus subject areas

Environmental Chemistry
Aquatic Science

More information

10.1016/j.fsi.2019.01.042

Cite this

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title = "Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish",

abstract = "Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.",

keywords = "Bacteria infection, Combined material, Immunostimulant, Nanoparticles",

author = "Xia, {Ivan Fan} and Cheung, {Jacky ST} and Manhui Wu and Wong, {Kwong Sen} and Kong, {Hang Kin} and Zheng, {Xiao Ting} and Wong, {Ka Hing} and Kwok, {Kevin WH}",

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AU - Xia, Ivan Fan

AU - Cheung, Jacky ST

AU - Wu, Manhui

AU - Wong, Kwong Sen

AU - Kong, Hang Kin

AU - Zheng, Xiao Ting

AU - Wong, Ka Hing

AU - Kwok, Kevin WH

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.

AB - Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.

KW - Bacteria infection

KW - Combined material

KW - Immunostimulant

KW - Nanoparticles

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DO - 10.1016/j.fsi.2019.01.042

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SN - 1050-4648

VL - 87

SP - 449

EP - 459

JO - Fish and Shellfish Immunology

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ER -

Dietary chitosan-selenium nanoparticle (CTS-SeNP) enhance immunity and disease resistance in zebrafish

Abstract

Keywords

ASJC Scopus subject areas

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