Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing

Zhijie Huan; Kar Hang Chu; Jie Yang; Dong Sun

doi:10.1109/NANO.2016.7751549

Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing

Zhijie Huan, Kar Hang Chu, Jie Yang, Dong Sun

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

This paper presents a new technique of fabricating 3D-printed scaffolds that can utilizes dielectrophoresis (DEP) for cell patterning. The scaffold was first fabricated using a 3D printer with a biodegradable polymer, polylactic acid (PLA). The electrical conductivity of the polymeric scaffold was enhanced through sputtering a thin layer of gold. When a voltage was supplied to the scaffold, non-uniform electric fields were generated so that cells were polarized and patterned onto the scaffold. Experiments were conducted to demonstrate that the gold-coated PLA scaffold could be used for rapid patterning MC3T3-E1 cells via DEP. Cell proliferation was assessed by (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT method and the result confirms the DEP cell patterning mechanism is not cytotoxic.

Original language	English
Title of host publication	16th International Conference on Nanotechnology - IEEE NANO 2016
Publisher	IEEE
Pages	853-856
Number of pages	4
ISBN (Electronic)	9781509039142
DOIs	https://doi.org/10.1109/NANO.2016.7751549
Publication status	Published - 21 Nov 2016
Event	16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 - Sendai, Japan Duration: 22 Aug 2016 → 25 Aug 2016

Conference

Conference	16th IEEE International Conference on Nanotechnology - IEEE NANO 2016
Country/Territory	Japan
City	Sendai
Period	22/08/16 → 25/08/16

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

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10.1109/NANO.2016.7751549

Cite this

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title = "Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing",

abstract = "This paper presents a new technique of fabricating 3D-printed scaffolds that can utilizes dielectrophoresis (DEP) for cell patterning. The scaffold was first fabricated using a 3D printer with a biodegradable polymer, polylactic acid (PLA). The electrical conductivity of the polymeric scaffold was enhanced through sputtering a thin layer of gold. When a voltage was supplied to the scaffold, non-uniform electric fields were generated so that cells were polarized and patterned onto the scaffold. Experiments were conducted to demonstrate that the gold-coated PLA scaffold could be used for rapid patterning MC3T3-E1 cells via DEP. Cell proliferation was assessed by (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT method and the result confirms the DEP cell patterning mechanism is not cytotoxic.",

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Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing. / Huan, Zhijie; Chu, Kar Hang; Yang, Jie et al.
16th International Conference on Nanotechnology - IEEE NANO 2016. IEEE, 2016. p. 853-856 7751549.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

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Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing

Abstract

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