Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing

Zhijie Huan, Kar Hang Chu, Jie Yang, Dong Sun

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review


This paper presents a new technique of fabricating 3D-printed scaffolds that can utilizes dielectrophoresis (DEP) for cell patterning. The scaffold was first fabricated using a 3D printer with a biodegradable polymer, polylactic acid (PLA). The electrical conductivity of the polymeric scaffold was enhanced through sputtering a thin layer of gold. When a voltage was supplied to the scaffold, non-uniform electric fields were generated so that cells were polarized and patterned onto the scaffold. Experiments were conducted to demonstrate that the gold-coated PLA scaffold could be used for rapid patterning MC3T3-E1 cells via DEP. Cell proliferation was assessed by (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT method and the result confirms the DEP cell patterning mechanism is not cytotoxic.
Original languageEnglish
Title of host publication16th International Conference on Nanotechnology - IEEE NANO 2016
Number of pages4
ISBN (Electronic)9781509039142
Publication statusPublished - 21 Nov 2016
Event16th IEEE International Conference on Nanotechnology - IEEE NANO 2016 - Sendai, Japan
Duration: 22 Aug 201625 Aug 2016


Conference16th IEEE International Conference on Nanotechnology - IEEE NANO 2016

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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