Dielectrophoresis-based automatic 3D cell manipulation and patterning through a micro-electrode integrated multi-layer scaffold

Kar Hang Chu, Zhijie Huan, James K. Mills, Jie Yang, Dong Sun

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

8 Citations (Scopus)

Abstract

Automatic manipulation and patterning of biological cells into an artificial scaffold is an imperative step in the production of high-quality tissue for tissue transplantation. This paper examines the incorporation of dielectrophoresis into a three-dimensional (3D) scaffold body for batch manipulation and patterning of cells. To facilitate dielectrophoresis-based manipulation, a multi-layer biocompatible scaffold structure utilizing its body as the integrated micro-electrodes was designed and fabricated using soft lithography. Voltage of opposite polarity was applied to the scaffold structure and the resultant electric field from the scaffold body polarized the cells in the culture medium and attracted them to migrate towards the scaffold body. Experiments were conducted and the results confirm that the proposed multi-layer scaffold is capable of generating dielectrophoretic forces to manipulate the cells to the scaffold surface, forming a three-dimensional cellular pattern automatically.
Original languageEnglish
Title of host publicationIROS 2014 Conference Digest - IEEE/RSJ International Conference on Intelligent Robots and Systems
PublisherIEEE
Pages2003-2008
Number of pages6
ISBN (Electronic)9781479969340
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes
Event2014 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2014 - Palmer House Hilton Hotel, Chicago, United States
Duration: 14 Sep 201418 Sep 2014

Conference

Conference2014 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2014
CountryUnited States
CityChicago
Period14/09/1418/09/14

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Software
  • Computer Vision and Pattern Recognition
  • Computer Science Applications

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