Diagnosis-on-a-chip: A microfluidic platform for cell culture and virus assays

Xuming Zhang, R. Chua, P. H. Yap, A. Q. Liu

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

1 Citation (Scopus)

Abstract

A high-throughput microfluidic chip for cell culture and immunofluorescence virus assays is presented in this paper. The chip is fabricated by laser engraving technology with the polymer material polymethylmethacrylate (PMMA). Madin-Darby canine kidney (MDCK) cell line has been successfully loaded, cultured and passaged in culturing chambers with 1mm diameter and 1.5 µl volume; and rapid growth of influenza A virus (H3N2) in MDCK cells cultured has been observed and proved by immunoflorescence assay. This kind of cell culture array could offer a platform for virus growth testing for vaccine production and also is promising for early diagnosis and drug screening. in drug screening, bioinformatics, and quantitative cell biology.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages694-696
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: 7 Oct 200711 Oct 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/TerritoryFrance
CityParis
Period7/10/0711/10/07

Keywords

  • In situ observation
  • Influenza A
  • Lab on a chip
  • MDCK cells

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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