TY - JOUR
T1 - Dextran Sulfate Sodium Salt (DSS) induced enteritis in Orange-spotted grouper, Epinephelus coioides
AU - Yu, Dapeng
AU - Yang, Guanjian
AU - Xia, Hongli
AU - Gan, Zhen
AU - Wang, Zhiwen
AU - Xia, Liqun
AU - Kwok, Kevin WH
AU - Cai, Jia
AU - Lu, Yishan
N1 - Funding Information:
This work was supported by the the National Key Research and Development Project ( 2020YFD0900201 ), National Natural Science Foundation of China ( 32102827 , 31972818 , 31472302 ), China Postdoctoral Science Foundation ( 2019M662959 ), Guangdong Basic and Applied Basic Research Foundation ( 2019A1515110987 ).This work supported by special Funds for science technology innovation and industrial development of Shenzhen Dapeng New District (Grand No. KJYF202001-27 ), Zhongshan Social Welfare and Basic Research Project ( 210803114048631 , 2108031148611 ), Shenzhen Science and Technology Project ( KCXFZ20211020165547010 ), Project supported by Shenzhen Fundamental Research Program ( JCYJ20210324130014035 ).
Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/6
Y1 - 2023/6
N2 - The enteritis is a common disease in fish farming, but the pathogenesis is still not fully understood. The aim of the present study was to investigate the inducement of Dextran Sulfate Sodium Salt (DSS) intestinal inflammation on Orange-spotted grouper (Epinephelus coioides). The fish were challenged with 200 μl 3% DSS via oral irrigation and feeding, an appropriate dose based on the disease activity index of inflammation. The results indicated that the inflammatory responses induced by DSS were closely associated with the expression of pro-inflammatory cytokines including interleukin 1β (IL-1β), IL-8, IL16, IL-10 and tumor necrosis factor α (TNF-α), as well as NF-κB and myeloperoxidase (MPO) activity. At day5 after DSS treatment, the highest levels of all parameters were observed. Also, the severe intestinal lesions (intestinal villus fusion and shedding), strong inflammatory cell infiltration and microvillus effacement were seen through histological examination and SEM (scanning electronic microscopy) analysis. During the subsequent 18 days of the experimental period, the injured intestinal villi were gradually recovery. These data is beneficial to further investigate the pathogenesis of enteritis in farmed fish, which is helpful for the control of enteritis in aquaculture.
AB - The enteritis is a common disease in fish farming, but the pathogenesis is still not fully understood. The aim of the present study was to investigate the inducement of Dextran Sulfate Sodium Salt (DSS) intestinal inflammation on Orange-spotted grouper (Epinephelus coioides). The fish were challenged with 200 μl 3% DSS via oral irrigation and feeding, an appropriate dose based on the disease activity index of inflammation. The results indicated that the inflammatory responses induced by DSS were closely associated with the expression of pro-inflammatory cytokines including interleukin 1β (IL-1β), IL-8, IL16, IL-10 and tumor necrosis factor α (TNF-α), as well as NF-κB and myeloperoxidase (MPO) activity. At day5 after DSS treatment, the highest levels of all parameters were observed. Also, the severe intestinal lesions (intestinal villus fusion and shedding), strong inflammatory cell infiltration and microvillus effacement were seen through histological examination and SEM (scanning electronic microscopy) analysis. During the subsequent 18 days of the experimental period, the injured intestinal villi were gradually recovery. These data is beneficial to further investigate the pathogenesis of enteritis in farmed fish, which is helpful for the control of enteritis in aquaculture.
KW - DSS
KW - Enteritis
KW - Epinephelus coioides
UR - http://www.scopus.com/inward/record.url?scp=85153593259&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2023.108742
DO - 10.1016/j.fsi.2023.108742
M3 - Journal article
C2 - 37100309
AN - SCOPUS:85153593259
SN - 1050-4648
VL - 137
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
M1 - 108742
ER -