Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae

Jing Jing, Chi on Chan, Lijia Xu, Dengping Jin, Xinwei Cao, Kam Wah Mok, H. S. Parekh, Sibao Chen

Research output: Journal article publicationJournal articleAcademic researchpeer-review

38 Citations (Scopus)


Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses of bioactives within traditional Chinese medicine. A fingerprint provides detailed information, specific for any given herb, thus facilitating the quality control measures of a given traditional Chinese medicine. In this article, quality assessment of Radix Scrophulariae was achieved by using high performance liquid chromatography combining diode-array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS). Eight batches of sample obtained from different origins in China were used to establish the fingerprint and quantitative analyses. By comparing the retention times, UV and MS spectral data with reference standards, four characteristic peaks in the chromatograms were confirmed as corresponding to acetoside, angoroside C, cinnamic acid, and harpagoside. In addition, other two characteristic peaks were tentatively identified, following the literature interpretation of HPLC-ESI-MS and LC-MS/MS (affording structural information) to be sibirioside A and scrophuloside B4, respectively. The results indicated that the newly developed HPLC-DAD-MS fingerprint method would be suitable for quality control of Radix Scrophulariae.
Original languageEnglish
Pages (from-to)830-835
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number4
Publication statusPublished - 5 Dec 2011


  • Fingerprint
  • High-performance liquid chromatography
  • Mass spectrometry
  • Radix scrophulariae

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

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