Development of a novel quantum dots and graphene oxide based fret assay for rapid detection of inva gene of Salmonella

Jiubiao Guo, Edward W.C. Chan, Sheng Chen, Zhenling Zeng

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

� 2017 Guo, Chan, Chen and Zeng. A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of detection (LOD) of ~4 nM of the invA gene in 20 min. The developed system has the potential to be used for Salmonella detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.
Original languageEnglish
Article number8
JournalFrontiers in Microbiology
Volume8
Issue numberJAN
DOIs
Publication statusPublished - 17 Jan 2017

Keywords

  • FRET assay
  • Graphene oxide
  • InvA
  • Quantum dots
  • Rapid detection
  • Salmonella

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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