@article{4f6c264c3d68450180a5ebd35790a7dd,
title = "Development of a high specificity typing method for the detection of herpes simplex virus",
abstract = "Herpes disease is caused by Herpes simplex virus (HSV). It has become one of the global health problems. This paper reports a method for HSV type testing. First specific primers sequence for HSV-1 and HSV-2 were selected, designed, and synthesized. Then, these amplification products were proved by sequencing and analysis. Lastly, we optimized the reaction system and PCR reaction program by orthogonal design and sensitivity testing. Results showed that the lowest concentration in HSV-type testing is about 6.67 × 106 copies/ml. Moreover, the specificity of detection was very high. So, this method has very great potentials for HSV type testing in clinical practice.",
keywords = "HSV typing, HSV-1, HSV-2, orthogonal design, primer design",
author = "Zhu Chen and Kaixuan Zhao and Boyu Tan and Zengrui Tong and Ziyu He and Xiaofang Luo and Lei Cai and Hanming Wang and Leung, {Polly H.M.} and Chow, {Franklin Wang Ngai} and Hui Chen and Yan Deng",
note = "Funding Information: This work was supported by the grant from the National Natural Science Foundation of China (61901168), Hunan Provincial Natural Science Foundation of China (2022JJ30229), Educational project of Hunan Province of China (Nos 21B0526, 21B0736). Hunan Key Research and Development Program (No. 2021SK 2003). Funding Information: This work was supported by the grant from the National Natural Science Foundation of China (61901168), Hunan Provincial Natural Science Foundation of China (2022JJ30229), Educational project of Hunan Province of China (Nos 21B0526, 21B0736). Hunan Key Research and Development Program (No. 2021SK 2003). Publisher Copyright: Copyright {\textcopyright} 2022 Chen, Zhao, Tan, Tong, He, Luo, Cai, Wang, Leung, Chow, Chen and Deng.",
year = "2022",
month = aug,
day = "17",
doi = "10.3389/fbioe.2022.955713",
language = "English",
volume = "10",
journal = "Frontiers in Bioengineering and Biotechnology",
issn = "2296-4185",
publisher = "Frontiers Media SA",
}