Development and validation of an HPLC-DAD method for bis(12)-hupyridone and its application to a pharmacokinetic study

Hua Yu, Wen Ming Li, Man Chun Cheung, Zhong Zuo, Paul R. Carlier, Ze Ming Gu, Kelvin Chan, Min Huang, Yi Tao Wang, Yifan Han

Research output: Journal article publicationJournal articleAcademic researchpeer-review

2 Citations (Scopus)

Abstract

A rapid and simple method of high performance liquid chromatography with UV detection for the quantification of bis(12)-hupyridone in rat blood has been developed and validated. Chromatographic separation was carried out in an Agilent Extend C185 μm column (length, 250 mm; inner diameter, 4.6 mm) using a mixture of water-acetonitrile-trifluoroacetic acid (81:19:0.04, v/v/v) as the mobile phase at a flow rate of 1 mL/min, with detection at 229 nm. The method used for the bis(12)-hupyridone quantification showed linearity for concentration range of 0.1-7.5 μg/mL with r2= 0.9991. The limit of detection and quantification of this method were 0.05 μg/mL and 0.1 μg/mL, respectively. The intra- and inter-day variations of the analysis were less than 4.22% with standard errors less than 13.3%. The developed method was successfully applied to the pharmacokinetic study of bis(12)-hupyridone after intravenous administration of 5 mg/kg and intraperitoneal administration of 10 and 20 mg/kg in rats.
Original languageEnglish
Pages (from-to)410-414
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume49
Issue number2
DOIs
Publication statusPublished - 20 Feb 2009

Keywords

  • Alzheimer's disease
  • Bis(12)-hupyridone
  • HPLC-DAD
  • Pharmacokinetics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

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