Determination of refractive index for single living cell using integrated biochip

X. J. Liang, A. Q. Liu, Xuming Zhang, P. H. Yap, T. C. Ayi, H. S. Yoon

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

22 Citations (Scopus)

Abstract

This paper reports a novel method for measuring the effective refractive index (RI) of single living cell with a small integrated chip. This microchip is able to determine the RI of living cell in real time without extra requirements of fluorescence labeling and chemical treatments, offering low cost and high accuracy meanwhile. It might provide an efficient approach for diseases or cancer diagnosis. The measurement system integrates laser diode, microlenses, and microfluidic channels onto a monolithic chip. In the experiments, two standard polystyrene beads with nominal RIs are employed to calibrate the system and five types of cancerous cells are subsequently measured. The results indicate that the RI of the tested cells ranges from 1.392 to 1.401, which is larger than typical value 1.35-1.37 for normal cells.
Original languageEnglish
Title of host publicationTRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages1712-1715
Number of pages4
Volume2
DOIs
Publication statusPublished - 9 Nov 2005
Externally publishedYes
Event13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of
Duration: 5 Jun 20059 Jun 2005

Conference

Conference13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Country/TerritoryKorea, Republic of
CitySeoul
Period5/06/059/06/05

Keywords

  • External cavity laser
  • Lab-on-a-chip
  • Microfluidics
  • Microlens
  • Refractive index

ASJC Scopus subject areas

  • Engineering(all)

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