Detection of Matrix Metallopeptidase 13 for Monitoring Stem Cell Differentiation and Early Diagnosis of Osteoarthritis by Fluorescent Light-Up Probes with Aggregation-Induced Emission Characteristics

Jinming Li, Wayne Yukwai Lee, Tianyi Wu, Chris Waitung Leung, Jianbin Xu, Dexter Siuhong Wong, Rui Li, Gang Li, Ben Zhong Tang, Liming Bian

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)

Abstract

Osteoarthritis (OA) is the leading cause of chronic disability affecting the elderly. There is an acute demand for novel approaches to sensitively and specifically detect OA biomarkers in conjunction with traditional radiographic outcomes to facilitate early diagnosis and allow timely treatment. In this study, a novel strategy is introduced to detect the activity of matrix metallopeptidase 13 (MMP-13)—a key enzyme responsible for cartilage matrix degradation in OA with a synthesized molecular probe containing a hydrophilic MMP-13-sensitive peptide conjugated to an aggregation induced emission fluorogen (AIEgen). The MMP-13 cleaves the MMP-13 sensitive peptide and induces aggregation of the hydrophogic AIEgen residues resulting in the activation of a fluorescent signal. The results demonstrate that this probe can detect increasing MMP-13 activity, which is an important marker of osteogenic differentiation in living and differentiating stem cells. This allows easy and semi-quantitative assessment of the extent of stem cell differentiation. Furthermore, by administering this probe to diseased joints of rats with induced OA, the real-time detection of MMP-13 activity is demonstrated in the osteoarthritic knee joints of living animals. It is believed that the molecular probe is a promising tool for real-time detection of disease markers with high fluorescence contrast to aid the early diagnosis of OA.

Original languageEnglish
Article number1800010
JournalAdvanced Biosystems
Volume2
Issue number10
DOIs
Publication statusPublished - Oct 2018

Keywords

  • aggregation-induced emission
  • enzyme cleavage
  • enzyme sensitive peptide
  • matrix metalloproteinase 13
  • osteoarthritis detection

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology(all)

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