@article{686a21ab645248fc982ad11cd3c3f4ff,
title = "Design of Polymeric Gene Carriers for Effective Intracellular Delivery",
abstract = "Polymeric carriers have emerged as major non-viral alternatives for gene delivery due to their lower immunogenicity and pathogenicity. However, during intracellular delivery of these carriers, multiple barriers have to be overcome or the efficiency of gene delivery will be impeded. A thorough understanding of these cellular impediments is pivotal to optimizing the efficiency of polymer-based gene delivery. This review delineates the major barriers encountered during intracellular delivery of polyplexes and discusses possible molecular designs to overcome these barriers. Based on a review of the latest strategies to enhance the intracellular delivery process, we provide insights into the further development of polymeric carriers with enhanced efficiency in transfection.",
keywords = "cellular uptake, non-viral gene delivery, nucleocytoplasmic transport, polymeric vector, transfection efficiency",
author = "Lai, {Wing Fu} and Wong, {Wing Tak}",
note = "Funding Information: The authors acknowledge Yau-Foon Tsui, Guoan Wang, Guoxing Deng, Minjian Huang, Runyu Wu, Tengyao Xu, Xiaoxin Cai, Xiaowei Ma, and Xuanqin Peng for helpful comments and suggestions during the writing of the manuscript. This work is supported by grants from the Shenzhen Science and Technology Innovation Committee (JCYJ20170302144812937) and from the Natural Science Foundation of Shenzhen University (2017092). The authors regret that, owing to space and reference number limitations, some important studies could be cited only indirectly through recent reviews. Funding Information: The authors acknowledge Yau-Foon Tsui, Guoan Wang, Guoxing Deng, Minjian Huang, Runyu Wu, Tengyao Xu, Xiaoxin Cai, Xiaowei Ma, and Xuanqin Peng for helpful comments and suggestions during the writing of the manuscript. This work is supported by grants from the Shenzhen Science and Technology Innovation Committee ( JCYJ20170302144812937 ) and from the Natural Science Foundation of Shenzhen University ( 2017092 ). The authors regret that, owing to space and reference number limitations, some important studies could be cited only indirectly through recent reviews. Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",
year = "2018",
month = jul,
doi = "10.1016/j.tibtech.2018.02.006",
language = "English",
volume = "36",
pages = "713--728",
journal = "Trends in Biotechnology",
issn = "0167-7799",
publisher = "Elsevier Ltd",
number = "7",
}