Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides

Cheuk Fai Chow, Michael H.W. Lam, Hongyan Sui, Wai Yeung Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

21 Citations (Scopus)

Abstract

A competitive indicator displacement assay has been successfully developed for the ratiometric determination of sulfhydryl-containing amino acids and peptides using heterobimetallic donor-acceptor complexes as chemodosimetric ensembles. Chromotropic cis-[ML2(CN)2] (M = FeII, RuII, OsII; L = diimine) are used as signaling indicators and PtII(DMSO)Cl2acceptor moiety is used as the receptor for the sulfhydryl-containing analytes. A series of three heterobimetallic donor-acceptor complexes: cis-FeII(bpy)2[CN-PtII(DMSO)Cl2]2(1), cir-RuII(bpy)2[CN-PtII(DMSO)Cl2]2(2) and cis-OsII(bpy)2[CN- PtII(DMSO)Cl2]2(3) are synthesized and characterized by X-ray crystallography. All the three ensembles are able to produce specific colorimetric/fluorimetric responses to sulfhydryl-containing amino acids (cysteine, homocysteine and methionine) as well as the sulfhydryl-containing small peptide glutathione. The mechanism of the competitive displacement assay is evaluated by examining the thermodynamics of formation of the donor-acceptor linkage and adducts between the acceptor metal and the sulfhydryl-containing analytes as well as by systematic variation of the donor and acceptor metals in the chemodosimetric ensembles.
Original languageEnglish
Pages (from-to)475-484
Number of pages10
JournalDalton Transactions
Issue number3
DOIs
Publication statusPublished - 7 Feb 2005
Externally publishedYes

ASJC Scopus subject areas

  • Inorganic Chemistry

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