Design and microfabrication of microreactor array for plate reader for E. coli research

Gaozhe Cai, Shilun Feng, Yang Liu, Wenshuai Wu, Jingbo Zhang, Jianhan Lin, Ai Qun Liu

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

In this paper, one microreactor array chip being specified used on plate reader has been designed, fabricated and demonstrated. Separated microreactors can be generated to contain the chemicals for different reactions. 4-Methylumbelliferone (4-MU) with different concentrations were first used to test the chip and monitored by the commercial plate reader machine, which shows the linear relationship between the concentrations and fluorescent intensities. Furthermore, fluorescence detection of low concentrations of E. Coli (Escherichia coli) based on commercial β-d-Glucuronidase (GUS) assay were successfully performed using this microreactor array combined with machine learning.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages434-435
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - Oct 2020
Externally publishedYes
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 4 Oct 20209 Oct 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period4/10/209/10/20

Keywords

  • 4-Methylumbelliferone
  • E. Coli detection
  • Microreactor
  • β-d-Glucuronidase

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • General Chemistry
  • Control and Systems Engineering

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