Abstract
Introduction:
Myopia is a substantial public health problem, affecting 33% of individuals over the age of 12 years in the United States and more than 80% people in Hong Kong. During childhood, an active process named emmetropization adjusted the expanding eye length to foucsed image on the retina. Any failure of emmetropization, results in refractive errors. However, defocused image’s effect on retinal signaling that accounts for either emmetropia or refractive errors had remained elusive. The research is to determine if the defocused image could change the signaling of retinal ganglion cells(RGCs) in mouse.
Methods:
Alpha ganglion cells were recorded from adult C57BL/6J wild-type mice. A mono green organic light-emitting display (OLEDXL, Olightek, China; 800 × 600-pixel resolution, 85 Hz refresh rate) presented different spatial frequencies light bar generated by PsychoPy onto the photoreceptor layer. Whole-cell recordings were made with electrodes pulled to 5−7 MΩ resistance. Spike trains were recorded with Axoscope software, with sorting by using Off-line Sorter and NeuroExplorer software.
Conclusions
1: Defocused images changed the signaling of RGCs in the mouse retina. The process might be the first step to induce myopia development.
2: Some RGCs reponded differently to plus and minus defocused image.
3: Gap Junction might play an important function in RGCs response to defocused images.
Myopia is a substantial public health problem, affecting 33% of individuals over the age of 12 years in the United States and more than 80% people in Hong Kong. During childhood, an active process named emmetropization adjusted the expanding eye length to foucsed image on the retina. Any failure of emmetropization, results in refractive errors. However, defocused image’s effect on retinal signaling that accounts for either emmetropia or refractive errors had remained elusive. The research is to determine if the defocused image could change the signaling of retinal ganglion cells(RGCs) in mouse.
Methods:
Alpha ganglion cells were recorded from adult C57BL/6J wild-type mice. A mono green organic light-emitting display (OLEDXL, Olightek, China; 800 × 600-pixel resolution, 85 Hz refresh rate) presented different spatial frequencies light bar generated by PsychoPy onto the photoreceptor layer. Whole-cell recordings were made with electrodes pulled to 5−7 MΩ resistance. Spike trains were recorded with Axoscope software, with sorting by using Off-line Sorter and NeuroExplorer software.
Conclusions
1: Defocused images changed the signaling of RGCs in the mouse retina. The process might be the first step to induce myopia development.
2: Some RGCs reponded differently to plus and minus defocused image.
3: Gap Junction might play an important function in RGCs response to defocused images.
| Original language | English |
|---|---|
| Publication status | Published - 26 Jun 2018 |
| Event | The Federation of American Societies for Experimental Biology) Annual Meeting - , United States Duration: 24 Jun 2018 → 29 Jun 2018 |
Conference
| Conference | The Federation of American Societies for Experimental Biology) Annual Meeting |
|---|---|
| Country/Territory | United States |
| Period | 24/06/18 → 29/06/18 |
