Data on differentially expressed proteins in rock inhibitor-treated human trabecular meshwork cells using SWATH-based proteomics

Sze Wan Shan, Chi Wai Do, Thomas Chuen Lam, Hoi Lam Li, W. Daniel Stamer, Chi Ho To

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Rho-associated coiled coil-forming protein kinase (ROCK) inhibitors represent a novel class of anti-glaucoma drugs because of their ocular hypotensive effects. However, the underlying mechanisms responsible for lowering intraocular pressure (IOP) are not completely clear. The protein profile changes in primary human trabecular meshwork (TM) cells after two days treatment with a ROCK inhibitor were studied using label-free SWATH acquisition. These results provided significant data of key protein candidates underlying the effect of ROCK inhibitor. Using the sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive TM proteome library. All raw data generated from IDA and SWATH acquisitions were uploaded and published in the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01254).

Original languageEnglish
Article number105846
JournalData in Brief
Volume31
Early online date12 Jun 2020
DOIs
Publication statusPublished - Aug 2020

Keywords

  • glaucoma
  • Rock inhibitor
  • Swath
  • Trabecular meshwork

ASJC Scopus subject areas

  • General

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