Daily Changes in the Phosphoproteome of the Dinoflagellate Lingulodinium

Bolin Liu, Chun Lap Samuel Lo, Daniel P. Matton, B. Franz Lang, David Morse

Research output: Journal article publicationJournal articleAcademic researchpeer-review

10 Citations (Scopus)

Abstract

The dinoflagellate Lingulodinium has a large number of daily rhythms, many of which have no biochemical correlates. We examined the possibility that changes in protein phosphorylation may mediate some of the rhythmic changes by comparing proteins prepared from midday (LD6) and midnight (LD18) cultures. We used two different methods, one a 2D gel protocol in which phosphoproteins were identified after staining with ProQ Diamond, and the other an LC-MS/MS identification of tryptic phosphopeptides that had been purified by TiO2chromatography. Two differentially phosphorylated proteins, a light harvesting complex protein and Rad24, were identified using the 2D gel protocol. Six differentially phosphorylated proteins, a polyketide synthase, an uncharacterized transporter, a LIM (actin binding) domain and three RNA binding domain proteins, were identified using the phosphopeptide enrichment protocol. We conclude that changes in protein phosphorylation may underlie some of the rhythmic behavior of Lingulodinium.
Original languageEnglish
Pages (from-to)746-754
Number of pages9
JournalProtist
Volume163
Issue number5
DOIs
Publication statusPublished - 1 Sep 2012

Keywords

  • Dinoflagellate.
  • Diurnal rhythm
  • Phosphopeptide enrichment
  • Phosphoprotein
  • Two dimensional gel electrophoresis

ASJC Scopus subject areas

  • Microbiology

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