Critical role of mass spectrometry proteomics in tear biomarker discovery for multifactorial ocular diseases (Review)

Jessica Yuen Wuen Ma, Ying Hon Sze, Jing Fang Bian, Thomas Chuen Lam

Research output: Journal article publicationJournal articleAcademic researchpeer-review

29 Citations (Scopus)


The tear film is a layer of body fluid that maintains the homeostasis of the ocular surface. The superior accessibility of tears and the presence of a high concentration of functional proteins make tears a potential medium for the discovery of non‑invasive biomarkers in ocular diseases. Recent advances in mass spectrometry (MS) have enabled determination of an in‑depth proteome profile, improved sensitivity, faster acquisition speed, proven variety of acquisition methods, and identification of disease biomarkers previously lacking in the field of ophthalmology. The use of MS allows efficient discovery of tear proteins, generation of reproducible results, and, more importantly, determines changes of protein quantity and post‑translation modifications in microliter samples. The present review compared techniques for tear collection, sample preparation, and acquisition applied for the discovery of tear protein markers in normal subjects and multifactorial conditions, including dry eye syndrome, diabetic retinopathy, thyroid eye disease and primary open‑angle glaucoma, which require an early diagnosis for treatment. It also summarized the contribution of MS to early discovery by means of disease‑related protein markers in tear fluid and the potential for transformation of the tear MS‑based proteome to antibody‑based assay for future clinical application.

Original languageEnglish
Article number83
JournalInternational Journal of Molecular Medicine
Issue number5
Publication statusPublished - 18 Mar 2021


  • Biomarker
  • Diabetic retinopathy
  • Dry eye syndrome
  • Glaucoma
  • Mass spectrometry
  • Tears
  • Thyroid eye disease

ASJC Scopus subject areas

  • Genetics


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