Covalent immobilization of carbohydrates on sol-gel-coated microplates

Lan Zou, Hei Leung Pang, Pak Ho Chan, Zhi Shu Huang, Lian Quan Gu, Kwok Yin Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)

Abstract

Carbohydrate microarrays have attracted increasing attention in recent years because of their ability to monitor biologically important protein-carbohydrate interactions in a high-throughput manner. Here we have developed an effective approach to immobilizing intact carbohydrates directly on polystyrene microtiter plates coated with amine-functionalized sol-gel monolayers. Lectin binding was monitored by fluorescence spectroscopy using these covalent arrays of carbohydrates that contained six mono- and di-saccharides on the microplates. In addition, binding affinities of lectin to carbohydrates were also quantitatively analyzed by determining IC50 values of lectin-specific antibody with these arrays. Our results indicate that microplate-based carbohydrate arrays can be efficiently fabricated by covalent immobilization of intact carbohydrates on sol-gel-coated microplates. The microplate-based carbohydrate arrays can be applied for screening of protein-carbohydrate interactions in a high-throughput manner.
Original languageEnglish
Pages (from-to)1195-1200
Number of pages6
JournalAnalyst
Volume133
Issue number9
DOIs
Publication statusPublished - 26 Aug 2008

ASJC Scopus subject areas

  • Analytical Chemistry

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