Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

Jianyong Wu; A. J. Daugulis; P. Faulkner; M. F.A. Goosen

doi:10.1007/BF02439322

Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

Jianyong Wu
, A. J. Daugulis
, P. Faulkner
, M. F.A. Goosen

Research output: Journal article publication › Journal article › Academic research › peer-review

8 Citations (Scopus)

Abstract

The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.

Original language	English
Pages (from-to)	335-340
Number of pages	6
Journal	Biotechnology Techniques
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF02439322
Publication status	Published - 1 Jul 1992
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

More information

10.1007/BF02439322

Cite this

@article{2df3eff032dc4cd88bca062cce7d8f73,

title = "Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay",

abstract = "The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.",

author = "Jianyong Wu and Daugulis, \{A. J.\} and P. Faulkner and Goosen, \{M. F.A.\}",

year = "1992",

month = jul,

day = "1",

doi = "10.1007/BF02439322",

language = "English",

volume = "6",

pages = "335--340",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Springer Science and Business Media B.V.",

number = "4",

}

TY - JOUR

T1 - Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

AU - Wu, Jianyong

AU - Daugulis, A. J.

AU - Faulkner, P.

AU - Goosen, M. F.A.

PY - 1992/7/1

Y1 - 1992/7/1

N2 - The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.

AB - The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.

UR - https://www.scopus.com/pages/publications/51249166930

U2 - 10.1007/BF02439322

DO - 10.1007/BF02439322

M3 - Journal article

SN - 0951-208X

VL - 6

SP - 335

EP - 340

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 4

ER -

Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

Abstract

ASJC Scopus subject areas

More information

Other files and links

Fingerprint

Cite this