Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

Jianyong Wu; A. J. Daugulis; P. Faulkner; M. F.A. Goosen

doi:10.1007/BF02439322

Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

Jianyong Wu, A. J. Daugulis, P. Faulkner, M. F.A. Goosen

Research output: Journal article publication › Journal article › Academic research › peer-review

8 Citations (Scopus)

Abstract

The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.

Original language	English
Pages (from-to)	335-340
Number of pages	6
Journal	Biotechnology Techniques
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF02439322
Publication status	Published - 1 Jul 1992
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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10.1007/BF02439322

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title = "Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay",

abstract = "The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.",

author = "Jianyong Wu and Daugulis, {A. J.} and P. Faulkner and Goosen, {M. F.A.}",

year = "1992",

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AU - Wu, Jianyong

AU - Daugulis, A. J.

AU - Faulkner, P.

AU - Goosen, M. F.A.

PY - 1992/7/1

Y1 - 1992/7/1

N2 - The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.

AB - The influence of reagent concentrations and temperature on measurement of LDH in Sf-9 insect cell cultures was examined. The LDH activity detected showed a linear correlation to loss of cell viability determined with the dye exclusion method.

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U2 - 10.1007/BF02439322

DO - 10.1007/BF02439322

M3 - Journal article

SN - 0951-208X

VL - 6

SP - 335

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JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 4

ER -

Correlation of LDH activity with loss of insect cell viability: An assessment of the LDH assay

Abstract

ASJC Scopus subject areas

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