Cordyceps sinensis mycelium extract induces human premyelocytic leukemia cell apoptosis through mitochondrion pathway

Q.X. Zhang, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

19 Citations (Scopus)

Abstract

This study was to identify the signaling pathways for the induction of HL-60 cell apoptosis by Cordyceps sinensis mycelium extract (CSME). CSME at 25 μg/ml induced nuclear fragmentation and DNA degradation, two hallmark events of apoptosis, in the HL-60 cells within 12–24 hrs of treatment. Concomitantly, several major events in the mitochondrial signal pathway occurred, including the loss of MTP (ΔΨm), cytochrome c release into the cytoplasm, the decrease in Bcl-2 protein level, the translocation of Bax protein from cytoplasm into mitochondria, and the activation of caspase-2, −3, and −9, but caspase-8, the initiator caspase in the death receptor pathway, was not activated. These results suggest that CSME induces apoptosis in HL-60 cell through the mitochondrial pathway rather than the death receptor pathway.
Original languageEnglish
Pages (from-to)52-57
Number of pages6
JournalExperimental Biology and Medicine
Volume232
Issue number1
Publication statusPublished - 2007

Keywords

  • Cordyceps sinensis
  • HL-60 cell
  • Caspases
  • Mitochondria
  • Cytochrome c
  • Bcl-2 proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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