Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process

Ferdian Wirawan, Chieh Lun Cheng, Yung Chung Lo, Chun Yen Chen, Jo Shu Chang, Shao Yuan Leu, Duu Jong Lee

Research output: Journal article publicationJournal articleAcademic researchpeer-review

13 Citations (Scopus)


Bioethanol produced from lignocellulosic materials has been considered as one of the most promising fuels to replace fossil fuels. Immobilized yeasts or bacteria have been frequently used in continuous system due to its feasibility for repeated use with high biomass retention during the continuous process. In this study, continuous SHcF (separate hydrolysis and co-fermentation) and SScF (simultaneous saccharification and co-fermentation) were evaluated for ethanol production from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). In SHcF fermentation, the ethanol yield and productivity of 0.36 g ethanol/g cellulose (corresponding to 70.65% of theoretical yield) and 1.868 g/L/h were achieved. In contrast, SScF system resulted in an ethanol yield of 0.414 g ethanol/g cellulose (corresponding to 81.17% of theoretical yield) and ethanol productivity of 0.705 g/L/h. The performance of the two systems are compared and discussed.

Original languageEnglish
Article number114871
JournalApplied Energy
Publication statusPublished - 15 May 2020


  • Bioethanol
  • Co-fermentation
  • Immobilized cells
  • Lignocellulosic materials
  • Pichia stipitis
  • Zymomonas mobilis

ASJC Scopus subject areas

  • Building and Construction
  • Energy(all)
  • Mechanical Engineering
  • Management, Monitoring, Policy and Law

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