Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors

Yujiao Zhu, Ziyu Huang, Qingming Chen, Qian Wu, Xiaowen Huang, Pui Kin So, Liyang Shao, Zhongping Yao, Yanwei Jia, Zhaohui Li, Weixing Yu, Yi Yang, Aoqun Jian, Shengbo Sang, Wendong Zhang, Xuming Zhang (Corresponding Author)

Research output: Journal article publicationJournal articleAcademic researchpeer-review

57 Citations (Scopus)


Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 μmol g−1 RuBisCO min−1 from CO2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization.

Original languageEnglish
Article number4049
JournalNature Communications
Issue number1
Publication statusPublished - Sept 2019

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy


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