Construction of recombinant Escherichia coli strains for polyhydroxybutyrate production using soy waste as nutrient

Kui Hong, Chung Leung Yun, Yi Kwok Sui, Ho Law Kin, Wai Hung Lo, Hong Chua, Peter Hoi Fu Yu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

13 Citations (Scopus)

Abstract

Construction and comparison of recombinant Escherichia coli strains harboring the polyhydroxybutyrate (PHB) operon from Ralstonia eutropha using vectors possessing different promotors, as well as the production of PHB from soy waste by the recombinant strain, are reported. The lac promotor was the most efficient on expression of the phb operon among the three promotors studied: i.e., lac promotor, T7 promotor and the normal σ70promotor. The pKS/PHB was the most efficient plasmid for phb operon expression among the three plasmids used: i.e., pKS-, pAED4, and pJM9131. It was observed that isopropyl-β-D-thiogalactopyanoside was not required for the induction of the expression of phb operon. The cell dry wt and polyhydroxyalkanoate content by E. coli XL-1 Blue (pKS/PHB) were 3.025 g/L and 27.83%, respectively.
Original languageEnglish
Pages (from-to)381-390
Number of pages10
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume84-86
DOIs
Publication statusPublished - 1 Jan 2000

Keywords

  • Escherichia coli
  • Fourier- transform infrared
  • Lac promotor
  • Polyhydroxybutyrates
  • Soy waste
  • T7 promotor

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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