Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates

Yujie Wang, Lifang Ruan, Wai Hung Lo, Hong Chua, Hoi Fu Yu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

7 Citations (Scopus)

Abstract

Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2C1 and pBE2C1AB, containing one or two PHA synthse genes, respectively. The two plasmids were inserted into Bacillus subtilis DB104 to generate modified strains, B. subtilis/pBE2C1 and B. subtilis/pBE2C1AB. The two recombinants strains were subjected to fermentation and showed PHA accumulation, the first reported example of mcl-PHA production in B. subtilis. Gas Chromatography analysis identified the compound produced by B. subtilis/pBE2C1 to be a hydroxydecanoate-co-hydroxydodecanoate (HD-co-HDD) polymer whereas that produced by B. subtilis/pBE2C1AB was a hydroxybutyrate-co- hydroxydecanoate-co-hydroxydodecanoate (HB-HD-HDD) polymer.
Original languageEnglish
Pages (from-to)1015-1022
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume132
Issue number1-3
DOIs
Publication statusPublished - 2 Jun 2006

Keywords

  • Bacillus subtilis
  • Cloning and expression
  • P (HB-co-mclHA)
  • PHA synthase gene
  • Polyhydroxyalkanoates (PHAs)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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