Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody

Wah Cheuk, Kathy O Y Wong, Sze Chuen Cesar Wong, John K C Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

75 Citations (Scopus)


Rabbit monoclonal antibody (MAb), which has become available only recently, theoretically combines the advantage of the high affinity attributable to its rabbit origin and the high specificity due to its monoclonal nature. Since immunohistochemical demonstration of cyclin D1 is notoriously difficult, this study aims to assess whether a newly available rabbit MAb against cyclin D1 (SP4) can improve the consistency of immunostaining, especially for the diagnosis of mantle cell lymphoma (MCL). A total of 150 cases of lymphoproliferative lesions, including 30 cases of MCL, histologic mimickers of MCL, and various types of lymphomas and leukemias, were studied. Immunostaining was performed on formalin-fixed, paraffin-embedded tissue sections using a labeled streptavidin-biotin peroxidase system in an automated immunostainer. All cases of MCL expressed cyclin D1, with a higher median staining score (8 out of a maximum of 12) compared with mouse MAb DCS-6 (score 4). In addition, 2 of 15 cases of B-cell chronic lymphocytic leukemia (B-CLL), 3 of 12 cases of multiple myeloma, and 2 of 5 cases of hairy cell leukemia were also positive. Comparable staining results could also be achieved by an optimized manual staining protocol. This study thus confirms the superior performance of the rabbit MAb SP4, which should permit consistent immunostaining for cyclin D1 to be readily achieved. The value of cyclin D1 immunohistochemistry in the differential diagnosis of MCL from other low-grade B-cell lymphomas is also affirmed, but with the caveat that rare cases of B-CLL can also be cyclin D1 positive.
Original languageEnglish
Pages (from-to)801-807
Number of pages7
JournalAmerican Journal of Surgical Pathology
Issue number6
Publication statusPublished - 1 Jun 2004
Externally publishedYes


  • Chronic lymphocytic leukemia
  • Cyclin D1
  • Hairy cell leukemia
  • Immunohistochemistry
  • Mantle cell lymphoma
  • Multiple myeloma
  • Rabbit monoclonal antibody

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine


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