Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells

Xiaoyu Chen; Rui Li; Siu Hong Dexter Wong; Kongchang Wei; Miao Cui; Huaijun Chen; Yuanzhang Jiang; Boguang Yang; Pengchao Zhao; Jianbin Xu; Heng Chen; Chao Yin; Sien Lin; Wayne Yuk Wai Lee; Yihan Jing; Zhen Li; Zhengmeng Yang; Jiang Xia; Guosong Chen; Gang Li; Liming Bian

doi:10.1038/s41467-019-10640-z

Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells

Xiaoyu Chen, Rui Li, Siu Hong Dexter Wong, Kongchang Wei, Miao Cui, Huaijun Chen, Yuanzhang Jiang, Boguang Yang, Pengchao Zhao, Jianbin Xu, Heng Chen, Chao Yin, Sien Lin, Wayne Yuk Wai Lee, Yihan Jing, Zhen Li, Zhengmeng Yang, Jiang Xia, Guosong Chen, Gang LiLiming Bian

Research output: Journal article publication › Journal article › Academic research › peer-review

65 Citations (Scopus)

Abstract

Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.

Original language	English
Article number	2705
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-10640-z
Publication status	Published - 1 Dec 2019

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/s41467-019-10640-z

http://hdl.handle.net/10397/88225

Cite this

Chen, X., Li, R., Wong, S. H. D., Wei, K., Cui, M., Chen, H., Jiang, Y., Yang, B., Zhao, P., Xu, J., Chen, H., Yin, C., Lin, S., Lee, W. Y. W., Jing, Y., Li, Z., Yang, Z., Xia, J., Chen, G., ... Bian, L. (2019). Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells. Nature Communications, 10(1), Article 2705. https://doi.org/10.1038/s41467-019-10640-z

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title = "Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells",

abstract = "Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.",

author = "Xiaoyu Chen and Rui Li and Wong, {Siu Hong Dexter} and Kongchang Wei and Miao Cui and Huaijun Chen and Yuanzhang Jiang and Boguang Yang and Pengchao Zhao and Jianbin Xu and Heng Chen and Chao Yin and Sien Lin and Lee, {Wayne Yuk Wai} and Yihan Jing and Zhen Li and Zhengmeng Yang and Jiang Xia and Guosong Chen and Gang Li and Liming Bian",

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doi = "10.1038/s41467-019-10640-z",

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Chen, X, Li, R, Wong, SHD, Wei, K, Cui, M, Chen, H, Jiang, Y, Yang, B, Zhao, P, Xu, J, Chen, H, Yin, C, Lin, S, Lee, WYW, Jing, Y, Li, Z, Yang, Z, Xia, J, Chen, G, Li, G & Bian, L 2019, 'Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells', Nature Communications, vol. 10, no. 1, 2705. https://doi.org/10.1038/s41467-019-10640-z

TY - JOUR

T1 - Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells

AU - Chen, Xiaoyu

AU - Li, Rui

AU - Wong, Siu Hong Dexter

AU - Wei, Kongchang

AU - Cui, Miao

AU - Chen, Huaijun

AU - Jiang, Yuanzhang

AU - Yang, Boguang

AU - Zhao, Pengchao

AU - Xu, Jianbin

AU - Chen, Heng

AU - Yin, Chao

AU - Lin, Sien

AU - Lee, Wayne Yuk Wai

AU - Jing, Yihan

AU - Li, Zhen

AU - Yang, Zhengmeng

AU - Xia, Jiang

AU - Chen, Guosong

AU - Li, Gang

AU - Bian, Liming

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.

AB - Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.

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JO - Nature Communications

JF - Nature Communications

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ER -

Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells

Abstract

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