Conformational manipulation of scale-up prepared single-chain polymeric nanogels for multiscale regulation of cells

Xiaoyu Chen, Rui Li, Siu Hong Dexter Wong, Kongchang Wei, Miao Cui, Huaijun Chen, Yuanzhang Jiang, Boguang Yang, Pengchao Zhao, Jianbin Xu, Heng Chen, Chao Yin, Sien Lin, Wayne Yuk Wai Lee, Yihan Jing, Zhen Li, Zhengmeng Yang, Jiang Xia, Guosong Chen, Gang LiLiming Bian

Research output: Journal article publicationJournal articleAcademic researchpeer-review

38 Citations (Scopus)

Abstract

Folded single chain polymeric nano-objects are the molecular level soft material with ultra-small size. Here, we report an easy and scalable method for preparing single-chain nanogels (SCNGs) with improved efficiency. We further investigate the impact of the dynamic molecular conformational change of SCNGs on cellular interactions from molecular to bulk scale. First, the supramolecular unfoldable SCNGs efficiently deliver siRNAs into stem cells as a molecular drug carrier in a conformation-dependent manner. Furthermore, the conformation changes of SCNGs enable dynamic and precise manipulation of ligand tether structure on 2D biomaterial interfaces to regulate the ligand–receptor ligation and mechanosensing of cells. Lastly, the dynamic SCNGs as the building blocks provide effective energy dissipation to bulk biomaterials such as hydrogels, thereby protecting the encapsulated stem cells from deleterious mechanical shocks in 3D matrix. Such a bottom-up molecular tailoring strategy will inspire further applications of single-chain nano-objects in the biomedical area.

Original languageEnglish
Article number2705
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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