Comparative Analysis of Asian Carps Parvalbumin Reveals the Evolutionary Divergence Pattern of a Major Fish Allergen

Judy Kin Wing Ng, Qing Xiong, Ling Shi, Christine Yee Yan Wai, Soo Kyung Shin, Fu Kiu Ao, Agnes Sze Yin Leung, Nicki Yat Hin Leung, Ting Fan Leung, Stephen Kwok Wing Tsui

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Background: Asian carps, a popular freshwater fish globally, are valued for their flavor and serve as a crucial protein source, especially for infants. However, grass carp parvalbumin is highly allergenic, surpassing the allergenicity of fish like salmon and cod. The allergenic potential of parvalbumin in other Asian carps remains unknown, underscoring the
need for allergen identification to improve the precision of fish allergy diagnosis and treatment.
Objectives: To identify all parvalbumin homologs in Asian carps and investigate the role of gene divergence in allergenic homolog formation.
Method: Three annotated genomes of Asian carp, including grass carp, black carp and bighead carp, were constructed using a hybrid assembly approach. Through sequence homology at the genomic level, all the homologs of major fish allergens were identified. Bioinformatics tools were then employed to reveal the gene structures, expression levels, and
protein conformations of parvalbumin.
Results: Grass carp genome analysis showed nine parvalbumin homologs, with Cid_PV2 most similar to Cten i 1. Bighead and black carp genomes had ten homologs, including potentially allergenic Mpi_PV7 and Hno_PV7. Tissue-specific expression patterns revealed alternative usage of parvalbumin homologs. Gene duplication events expanded parvalbumin copies in bony fish, with two gene clusters identified in Asian carp genomes.
Conclusion: All the homologs of Asian carps’ parvalbumin were accurately identified and gene divergence contributed to the formation of allergenic homologs. Together with a comprehensive gene sequence profile of carps’ parvalbumin, those could be applied to achieve a more precise clinical diagnostic test.
Original languageEnglish
JournalAsian Pacific Journal of Allergy and Immunology
DOIs
Publication statusPublished - 19 May 2024

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