Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation

Zhehui Feng, Jau Shyong Hong, Qiping Qi, Yifan Han, Belinda Wilson, Michael Iadarola, Ningyi Tiao, Guoying Bing

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.
Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalNeuroscience Letters
Volume296
Issue number2-3
DOIs
Publication statusPublished - 22 Dec 2000
Externally publishedYes

Keywords

  • Cloning
  • Fos-related antigen
  • Gel-shift
  • Gene
  • Guanosine triphosphate
  • Immunoscreening
  • Transcription factors

ASJC Scopus subject areas

  • Neuroscience(all)

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