Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation

Zhehui Feng; Jau Shyong Hong; Qiping Qi; Yifan Han; Belinda Wilson; Michael Iadarola; Ningyi Tiao; Guoying Bing

doi:10.1016/S0304-3940(00)01641-4

Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation

Zhehui Feng, Jau Shyong Hong, Qiping Qi, Yifan Han, Belinda Wilson, Michael Iadarola, Ningyi Tiao, Guoying Bing

Research output: Journal article publication › Journal article › Academic research › peer-review

Abstract

C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	129-132
Number of pages	4
Journal	Neuroscience Letters
Volume	296
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(00)01641-4
Publication status	Published - 22 Dec 2000
Externally published	Yes

Keywords

Cloning
Fos-related antigen
Gel-shift
Gene
Guanosine triphosphate
Immunoscreening
Transcription factors

ASJC Scopus subject areas

General Neuroscience

More information

10.1016/S0304-3940(00)01641-4

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title = "Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation",

abstract = "C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Cloning, Fos-related antigen, Gel-shift, Gene, Guanosine triphosphate, Immunoscreening, Transcription factors",

author = "Zhehui Feng and Hong, {Jau Shyong} and Qiping Qi and Yifan Han and Belinda Wilson and Michael Iadarola and Ningyi Tiao and Guoying Bing",

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AU - Feng, Zhehui

AU - Hong, Jau Shyong

AU - Qi, Qiping

AU - Han, Yifan

AU - Wilson, Belinda

AU - Iadarola, Michael

AU - Tiao, Ningyi

AU - Bing, Guoying

PY - 2000/12/22

Y1 - 2000/12/22

N2 - C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.

AB - C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. (C) 2000 Elsevier Science Ireland Ltd.

KW - Cloning

KW - Fos-related antigen

KW - Gel-shift

KW - Gene

KW - Guanosine triphosphate

KW - Immunoscreening

KW - Transcription factors

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U2 - 10.1016/S0304-3940(00)01641-4

DO - 10.1016/S0304-3940(00)01641-4

M3 - Journal article

C2 - 11108998

SN - 0304-3940

VL - 296

SP - 129

EP - 132

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation

Abstract

Keywords

ASJC Scopus subject areas

More information

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