Cloning and characterization of a novel Krüppel-like zinc finger gene, ZNF268, expressed in early human embryo

D. M. Gou; Y. Sun; L. Gao; Ming Cheung Chow; J. Huang; Y. D. Feng; D. H. Jiang; W. X. Li

doi:10.1016/S0167-4781(01)00194-4

Cloning and characterization of a novel Krüppel-like zinc finger gene, ZNF268, expressed in early human embryo

D. M. Gou, Y. Sun, L. Gao, Ming Cheung Chow, J. Huang, Y. D. Feng, D. H. Jiang, W. X. Li

Research output: Journal article publication › Journal article › Academic research › peer-review

30 Citations (Scopus)

Abstract

With the aim of identifying genes involved in early human embryonic development, we have isolated a cDNA clone representing a novel human zinc finger gene ZNF268 from 3 week old human embryo cDNA library using a differential hybridization strategy. The complete cDNA sequence of ZNF268 contained an open reading frame of 2841 nucleotides that encodes a 947 amino acid protein with an N-terminal Krüppel-associated box (KRAB) domain and 24 C-terminal zinc fingers. Northern blot analysis showed that ZNF268 mRNA is mainly expressed in 3-5 week old human embryos suggesting it could play certain roles in the embryogenesis. The gene consists of six exons spanning about 22 kb of genomic DNA. According to the genomic sequence from the HTGS database, the ZNF268 gene is assigned to human chromosome 5.

Original language	English
Pages (from-to)	306-310
Number of pages	5
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1518
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(01)00194-4
Publication status	Published - 16 Apr 2001

Keywords

cDNA
Embryo
Genome structure
Human
KRAB
Zinc finger protein

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

More information

10.1016/S0167-4781(01)00194-4

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title = "Cloning and characterization of a novel Kr{\"u}ppel-like zinc finger gene, ZNF268, expressed in early human embryo",

abstract = "With the aim of identifying genes involved in early human embryonic development, we have isolated a cDNA clone representing a novel human zinc finger gene ZNF268 from 3 week old human embryo cDNA library using a differential hybridization strategy. The complete cDNA sequence of ZNF268 contained an open reading frame of 2841 nucleotides that encodes a 947 amino acid protein with an N-terminal Kr{\"u}ppel-associated box (KRAB) domain and 24 C-terminal zinc fingers. Northern blot analysis showed that ZNF268 mRNA is mainly expressed in 3-5 week old human embryos suggesting it could play certain roles in the embryogenesis. The gene consists of six exons spanning about 22 kb of genomic DNA. According to the genomic sequence from the HTGS database, the ZNF268 gene is assigned to human chromosome 5.",

keywords = "cDNA, Embryo, Genome structure, Human, KRAB, Zinc finger protein",

author = "Gou, {D. M.} and Y. Sun and L. Gao and Chow, {Ming Cheung} and J. Huang and Feng, {Y. D.} and Jiang, {D. H.} and Li, {W. X.}",

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AU - Gou, D. M.

AU - Sun, Y.

AU - Gao, L.

AU - Chow, Ming Cheung

AU - Huang, J.

AU - Feng, Y. D.

AU - Jiang, D. H.

AU - Li, W. X.

PY - 2001/4/16

Y1 - 2001/4/16

N2 - With the aim of identifying genes involved in early human embryonic development, we have isolated a cDNA clone representing a novel human zinc finger gene ZNF268 from 3 week old human embryo cDNA library using a differential hybridization strategy. The complete cDNA sequence of ZNF268 contained an open reading frame of 2841 nucleotides that encodes a 947 amino acid protein with an N-terminal Krüppel-associated box (KRAB) domain and 24 C-terminal zinc fingers. Northern blot analysis showed that ZNF268 mRNA is mainly expressed in 3-5 week old human embryos suggesting it could play certain roles in the embryogenesis. The gene consists of six exons spanning about 22 kb of genomic DNA. According to the genomic sequence from the HTGS database, the ZNF268 gene is assigned to human chromosome 5.

AB - With the aim of identifying genes involved in early human embryonic development, we have isolated a cDNA clone representing a novel human zinc finger gene ZNF268 from 3 week old human embryo cDNA library using a differential hybridization strategy. The complete cDNA sequence of ZNF268 contained an open reading frame of 2841 nucleotides that encodes a 947 amino acid protein with an N-terminal Krüppel-associated box (KRAB) domain and 24 C-terminal zinc fingers. Northern blot analysis showed that ZNF268 mRNA is mainly expressed in 3-5 week old human embryos suggesting it could play certain roles in the embryogenesis. The gene consists of six exons spanning about 22 kb of genomic DNA. According to the genomic sequence from the HTGS database, the ZNF268 gene is assigned to human chromosome 5.

KW - cDNA

KW - Embryo

KW - Genome structure

KW - Human

KW - KRAB

KW - Zinc finger protein

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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Cloning and characterization of a novel Krüppel-like zinc finger gene, ZNF268, expressed in early human embryo

Abstract

Keywords

ASJC Scopus subject areas

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