TY - JOUR
T1 - Chelator-Free Radiolabeling of Nanographene: Breaking the Stereotype of Chelation
AU - Shi, Sixiang
AU - Xu, Cheng
AU - Yang, Kai
AU - Goel, Shreya
AU - Valdovinos, Hector F.
AU - Luo, Haiming
AU - Ehlerding, Emily B.
AU - England, Christopher G.
AU - Cheng, Liang
AU - Chen, Feng
AU - Nickles, Robert J.
AU - Liu, Zhuang
AU - Cai, Weibo
N1 - Funding Information:
This work is supported, in part, by the University of Wisconsin?Madison, the National Institutes of Health (NIBIB/NCI 1R01CA169365, 1R01EB021336, P30CA014520, T32CA009206 and T32GM008505), the American Cancer Society (125246-RSG-13-099-01-CCE), and Grainger Foundation (Wisconsin Distinguished Graduate Fellowship). We also gratefully acknowledge the Analytical Instrumentation Center of the School of Pharmacy at University of Wisconsin?Madison for obtaining FTIR spectra.
Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2017/3/6
Y1 - 2017/3/6
N2 - Macrocyclic chelators have been widely employed in the realm of nanoparticle-based positron emission tomography (PET) imaging, whereas its accuracy remains questionable. Here, we found that 64Cu can be intrinsically labeled onto nanographene based on interactions between Cu and the π electrons of graphene without the need of chelator conjugation, providing a promising alternative radiolabeling approach that maintains the native in vivo pharmacokinetics of the nanoparticles. Due to abundant π bonds, reduced graphene oxide (RGO) exhibited significantly higher labeling efficiency in comparison with graphene oxide (GO) and exhibited excellent radiostability in vivo. More importantly, nonspecific attachment of 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) on nanographene was observed, which revealed that chelator-mediated nanoparticle-based PET imaging has its inherent drawbacks and can possibly lead to erroneous imaging results in vivo.
AB - Macrocyclic chelators have been widely employed in the realm of nanoparticle-based positron emission tomography (PET) imaging, whereas its accuracy remains questionable. Here, we found that 64Cu can be intrinsically labeled onto nanographene based on interactions between Cu and the π electrons of graphene without the need of chelator conjugation, providing a promising alternative radiolabeling approach that maintains the native in vivo pharmacokinetics of the nanoparticles. Due to abundant π bonds, reduced graphene oxide (RGO) exhibited significantly higher labeling efficiency in comparison with graphene oxide (GO) and exhibited excellent radiostability in vivo. More importantly, nonspecific attachment of 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) on nanographene was observed, which revealed that chelator-mediated nanoparticle-based PET imaging has its inherent drawbacks and can possibly lead to erroneous imaging results in vivo.
KW - graphene
KW - imaging agents
KW - nanoparticles
KW - positron emission tomography
KW - radiolabeling
UR - http://www.scopus.com/inward/record.url?scp=85012034056&partnerID=8YFLogxK
U2 - 10.1002/anie.201610649
DO - 10.1002/anie.201610649
M3 - Journal article
C2 - 28170126
AN - SCOPUS:85012034056
SN - 1433-7851
VL - 56
SP - 2889
EP - 2892
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 11
ER -