Chelator-Free Labeling of Layered Double Hydroxide Nanoparticles for in Vivo PET Imaging

Sixiang Shi, Brianne C. Fliss, Zi Gu, Yian Zhu, Hao Hong, Hector F. Valdovinos, Reinier Hernandez, Shreya Goel, Haiming Luo, Feng Chen, Todd E. Barnhart, Robert J. Nickles, Zhi Ping Xu, Weibo Cai

Research output: Journal article publicationJournal articleAcademic researchpeer-review

53 Citations (Scopus)


Layered double hydroxide (LDH) nanomaterial has emerged as a novel delivery agent for biomedical applications due to its unique structure and properties. However, in vivo positron emission tomography (PET) imaging with LDH nanoparticles has not been achieved. The aim of this study is to explore chelator-free labeling of LDH nanoparticles with radioisotopes for in vivo PET imaging. Bivalent cation 64 Cu2+ and trivalent cation 44 Sc3+ were found to readily label LDH nanoparticles with excellent labeling efficiency and stability, whereas tetravalent cation 89 Zr4+ could not label LDH since it does not fit into the LDH crystal structure. PET imaging shows that prominent tumor uptake was achieved in 4T1 breast cancer with 64 Cu-LDH-BSA via passive targeting alone (7.7 ± 0.1%ID/g at 16 h post-injection; n = 3). These results support that LDH is a versatile platform that can be labeled with various bivalent and trivalent radiometals without comprising the native properties, highly desirable for PET image-guided drug delivery.

Original languageEnglish
Article number16930
JournalScientific Reports
Publication statusPublished - 20 Nov 2015
Externally publishedYes

ASJC Scopus subject areas

  • General


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