TY - GEN
T1 - Characterization of a Microchip Device for Cell Patterning via Negative Dielectrophoresis
AU - Huang, Kaicheng
AU - Chu, Henry K.
AU - Lu, Bo
AU - Cheng, Li
PY - 2018/7/2
Y1 - 2018/7/2
N2 - Patterning cells on a substrate is useful for cell assay and characterization. In this paper, a microchip employing negative dielectrophoresis was designed for efficient cell pattering. This chip consists of a 4-by-4 dot electrode array and the cell patterns are formed on a substrate lying on the microchip. To facilitate the microchip design, electric fields generated from the microchip were first simulated using software, and experiments were conducted to validate the performance of the microchip. Yeast cells suspending in the 6-aminohexanoic acid (AHA) solution were used in this study, and the effects of the cell medium, the voltage input and the voltage frequency were analyzed. The results confirm that cell patterns are successfully created with the microchip and this microchip offers an easy method to fabricate cell patterns on a substrate for characterization.
AB - Patterning cells on a substrate is useful for cell assay and characterization. In this paper, a microchip employing negative dielectrophoresis was designed for efficient cell pattering. This chip consists of a 4-by-4 dot electrode array and the cell patterns are formed on a substrate lying on the microchip. To facilitate the microchip design, electric fields generated from the microchip were first simulated using software, and experiments were conducted to validate the performance of the microchip. Yeast cells suspending in the 6-aminohexanoic acid (AHA) solution were used in this study, and the effects of the cell medium, the voltage input and the voltage frequency were analyzed. The results confirm that cell patterns are successfully created with the microchip and this microchip offers an easy method to fabricate cell patterns on a substrate for characterization.
UR - http://www.scopus.com/inward/record.url?scp=85064133518&partnerID=8YFLogxK
U2 - 10.1109/ROBIO.2018.8665210
DO - 10.1109/ROBIO.2018.8665210
M3 - Conference article published in proceeding or book
AN - SCOPUS:85064133518
T3 - 2018 IEEE International Conference on Robotics and Biomimetics, ROBIO 2018
SP - 1521
EP - 1526
BT - 2018 IEEE International Conference on Robotics and Biomimetics, ROBIO 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2018 IEEE International Conference on Robotics and Biomimetics, ROBIO 2018
Y2 - 12 December 2018 through 15 December 2018
ER -