Characteristics of single neurons cultured on microelectrode arrays in vitro for chemical sensing

Mo Yang, Xuan Zhang, Yu Zhang, Cengiz S. Ozkan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)

Abstract

In this paper, a single-neuron sensor was developed for chemical agent sensing. Single neurons were positioned over individual microelectrodes using positive dielectrophoretic traps. This enables the continuous extracellular electrophysiological measurements from individual neurons. A remarkably stable recording environment was obtained under the noninvasive conditions. The magnitude and temporal characteristics of an action potential recorded depended on sealing conditions. Then, consecutive sensing experiments were done to testify the stability and repeatability of this kind of single-neuron-based sensor. The responses of neuron-electrode sensor to chemical agent concentration change and to the consecutive experiments were also explored. This sensor exhibits a faster response with the increase of concentration and responds slowly during the consecutive experiments.
Original languageEnglish
Pages (from-to)690-695
Number of pages6
JournalIEEE Sensors Journal
Volume5
Issue number4
DOIs
Publication statusPublished - 1 Aug 2005
Externally publishedYes

Keywords

  • Bioelectronic noise
  • Cell-based biosensors
  • Extra-cellular signal
  • Ion channel

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

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